In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide
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منابع مشابه
In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide.
Bacterial collagenase was used to study the susceptibility of dermal sheep collagen (DSC) cross-linked with a mixture of the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide (E/N-DSC) towards enzymatic degradation. Contrary to non-cross-linked DSC (N-DSC), which had a rate of weight-loss of 18.1% per hour upon degradation, no weig...
متن کاملCross-linking of dermal sheep collagen using a water-soluble carbodiimide.
A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC). Cross-linking using EDC involves the activation of carboxylic acid groups to give O-acylisourea groups, which form cross-links after reaction with free amine groups. Treatment of dermal sheep collagen (DSC) with EDC (E-DSC)...
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Glutaraldehyde (GA) fixation of bioprosthetic tissue is a well adapted technique, with commercial products on the market for almost 40 years. Amine groups present in tissue react with GA to form different types of cross-links. An estimation of the degree of cross-linking of the tissue can be obtained by measuring the concentration of residual amine groups, which is frequently carried out with t...
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The influence of ethylene oxide gas treatment on the in vitro degradation behavior of noncrosslinked, glutaraldehyde crosslinked or hexamethylene diisocyanate crosslinked dermal sheep collagen (DSC) using bacterial collagenase is described. The results obtained were compared with the degradation behavior of either nonsterilized or gamma-sterilized DSC. Upon ethylene oxide sterilization, reactio...
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ژورنال
عنوان ژورنال: Biomaterials
سال: 1996
ISSN: 0142-9612
DOI: 10.1016/0142-9612(96)86737-8